anti phosphonf kb p65  (Cell Signaling Technology Inc)

Journal: Food & function

Article Title: Characterization of different stages of Maillard reaction in soy: impact on physicochemical properties and immunogenicity of soy proteins.

doi: 10.1039/d4fo04400b

Figure Lengend Snippet: Fig. 5 Expression of phosphorylated NFkB p65 shows an increased trend in macrophages after stimulation with glycated soy. Expression of phosphorylated NFkB P65, analyzed by western blot (A) THP-1 differen- tiated into macrophages, stimulated with 25 μg ml−1 glycated soy or 100 ng ml−1 LPS and 10 ng ml−1 IFNy for 10 minutes, data is representative of 3 replicates. (B) Primary derived adherent monocytes from one donor, stimulated with 25 μg ml−1 glycated soy or 100 ng ml−1 LPS and 10 ng ml−1 IFNy, data is representative of two replicates. Quantified data based on the adjusted volume normalized to beta actin. Data was processed and quantified using the ImageLab software from BioRad.

Article Snippet: The membrane was then washed 3×, followed by incubation with primary antibody (PhosphoNF-κB p65 (Ser536) (93H1) Rabbit mAb, Cell Signaling) overnight at 4 °C.

Techniques: Expressing, Western Blot, Derivative Assay, Software